種屬

人

別稱

SKNDZ

組織來(lái)源

大腦

疾病

神經(jīng)母細(xì)胞瘤細(xì)胞

傳代比例/細(xì)胞消化

1:2傳代,

完全培養(yǎng)基配置

DMEM培養(yǎng)基 ；10%胎牛血清 ；1%雙抗

簡(jiǎn)介

SK-N-DZ are neuroblasts that were isolated from the brain of a 2-year-old, White female patient with neuroblastoma.SK-N-DZ are neuroblasts that were isolated from the brain of a 2-year-old, White female patient with neuroblastoma. Expression of the N-myc gene product was reduced in differentiated SK-N-DZ cells as compared with undifferentiated cells. Expression of the c-src gene product, pp60c-src was enhanced in differentiated SK-N-DZ cells as was tyrosine phosphorylation of cellular proteins. The cells exhibit moderate MDR1 expression. Retinoic acid induces differentiation in this line.

形態(tài)

成神經(jīng)細(xì)胞樣

生長(zhǎng)特征

貼壁生長(zhǎng)

STR

Amelogenin X CSF1PO 12 D3S1358 17 D5S818 12 D7S820 12,13 D8S1179 12,13 D13S317 8,11

D16S539 9,11 D18S51 15,17 D21S11 29,31 FGA 18,22 Penta D 9,14 Penta E 7,10 TH01 6,9 TPOX 8

vWA 16,18

倍增時(shí)間

每周 2 至 3 次

培養(yǎng)條件

氣相：空氣，95%；二氧化碳，5%。 溫度：37攝氏度，培養(yǎng)箱濕度為70%-80%。

凍存條件

凍存液：90%FBS，DMSO 10%,

或使用非程序凍存液：官網(wǎng)貨號(hào)JY-H040

保藏機(jī)構(gòu)

ATCC; CRL-2149

備注

該細(xì)胞貼壁松散，收貨如有大塊脫落或全部脫落的細(xì)胞團(tuán)，為正?，F(xiàn)象，請(qǐng)按照收貨注意事項(xiàng)處理。

產(chǎn)品使用

僅限于科學(xué)研究，不可作為動(dòng)物或人類疾病的治療產(chǎn)品使用。